- will
amplify both
methylated and
unmethylated sequences, in
contrast to methylation-specific PCR. All
sites of
unmethylated cytosines are displa**** as thymines...
-
bases are
often improperly resolved to A:T;
whereas the
deamination of
unmethylated cytosine results in a uracil,
which as a
foreign base is
quickly replaced...
-
methylated as well as
unmethylated forms of
restriction sites. In contrast, the
other restriction enzyme can
recognize only the
unmethylated form of the restriction...
- targeted, and they are
characterized by the
alternation of
methylated and
unmethylated domains. High CpG
methylation in
mammalian genomes has an evolutionary...
-
Approximately 1 μg of
genomic DNA is used in
bisulfite conversion to
convert the
unmethylated cytosine into uracil. The
product contains unconverted cytosine where...
- In the
following decade, this
discovery led to the
revelation that
unmethylated cytosine reacted much
faster to
sodium bisulfite treatment than did 5-methylcytosine...
- with a
methylated MGMT
promoter have
longer survival than
those with an
unmethylated MGMT promoter, due in part to
increased sensitivity to temozolomide....
- in the
codon table. 5-Methylcytosine is more
prone to
transition than
unmethylated cytosine, due to
spontaneous deamination. This
mechanism is important...
- methylation-dependent
sequence differences.
During sodium bisulfite treatment,
unmethylated cytosine residues are
converted to uracil,
while methylated cytosine...
-
internalized from the
plasma membrane,
binds DNA (preferentially DNA
containing unmethylated CpGs of
bacterial or
viral origin), and
triggers signaling cascades that...
