- targeted, and they are
characterized by the
alternation of
methylated and
unmethylated domains. High CpG
methylation in
mammalian genomes has an evolutionary...
- will
amplify both
methylated and
unmethylated sequences, in
contrast to methylation-specific PCR. All
sites of
unmethylated cytosines are displa**** as thymines...
- In the
following decade, this
discovery led to the
revelation that
unmethylated cytosine reacted much
faster to
sodium bisulfite treatment than did 5-methylcytosine...
-
Approximately 1 μg of
genomic DNA is used in
bisulfite conversion to
convert the
unmethylated cytosine into uracil. The
product contains unconverted cytosine where...
-
bases are
often improperly resolved to A:T;
whereas the
deamination of
unmethylated cytosine results in a uracil,
which as a
foreign base is
quickly replaced...
-
methylated as well as
unmethylated forms of
restriction sites. In contrast, the
other restriction enzyme can
recognize only the
unmethylated form of the restriction...
- methylated, but the
cytosines near the
transcription start site were
unmethylated.
Methylation has been
shown to
functionally impact Sp1
sites as well...
- with a
methylated MGMT
promoter have
longer survival than
those with an
unmethylated MGMT promoter, due in part to
increased sensitivity to temozolomide....
- in the
codon table. 5-Methylcytosine is more
prone to
transition than
unmethylated cytosine, due to
spontaneous deamination. This
mechanism is important...
- methylation-dependent
sequence differences.
During sodium bisulfite treatment,
unmethylated cytosine residues are
converted to uracil,
while methylated cytosine...
