- the DNA
sequence encodes for the
position of
plectonemic supercoils. Furthermore, DNA
supercoils were
found to be
enriched at the
transcription start sites...
-
introduce negative supercoils into DNA at the
expense of ATP
hydrolysis is what
allows bacterial DNA to have free
negative supercoils. The
ability of gyrase...
-
Cozzarelli and co-workers. DNA
gyrase catalyzes the
introduction of
negative supercoils into DNA and is the only type II
enzyme to do this, all the
others catalyze...
-
existing negative supercoils before causing a
buildup of
positive supercoils ahead of RNAP and
introduces more
negative supercoils behind RNAP. In principle...
- cell is to
relax positive supercoils. It
shares this role with DNA gyrase,
which is also able to
relax positive supercoils. Together,
gyrase and topoisomerase...
-
whereas eukaryotic topoisomerase I (topo IB) can
introduce positive supercoils,
separating the DNA of
daughter chromosomes after DNA replication, and...
- transcription, for example, the
adjacent unopened DNA
winds tighter (
supercoils), like
opening the
middle of a
twisted rope. The
stress caused by this...
- result, the type I
enzyme removes supercoils from DNA one at a time,
whereas the type II
enzyme removes supercoils two at a time. The topo I of both prokaryotes...
-
strands of the DNA
helix simultaneously in
order to
manage DNA
tangles and
supercoils. They use the
hydrolysis of ATP,
unlike Type I topoisomerase. In this...
-
positive supercoils into DNA,
contrary to the
typical negative supercoils introduced by the type II
topoisomerase DNA gyrase.
These positive supercoils can...
