-
molecular biology,
subcloning is a
technique used to move a
particular DNA
sequence from a
parent vector to a
destination vector.
Subcloning is not to be confused...
- genomes.
Cancers usually show
tumour heterogeneity,
containing multiple subclones. They also
frequently have
reduced expression of DNA
repair enzymes due...
- may give rise to
subclones that
possess an
evolutionary advantage over the
others within the
tumour environment, and
these subclones may
become dominant...
-
reported in 1973. A neuroblast-like
subclone of SK-N-SH,
named SH-SY, was
subcloned as SH-SY5,
which was
subcloned a
third time to
produce the SH-SY5Y...
- strand. The
concept is used in
molecular biology, in cloning, or when
subcloning insert DNA into
vector DNA. Such ends may be
generated by restriction...
-
rates of mutation, the high
heterogeneity (variability) of
tumor cell
subclones, and the
absence of
genetic recombination.
Phylogenetics can also aid...
- T cloning) is a
subcloning technique that
avoids the use of
restriction enzymes and is
easier and
quicker than
traditional subcloning. The
technique relies...
- were
derived in 1961 by I. A.
Macpherson and M. G. P. Stoker. Nowadays,
subclone 13 is
occasionally used,
which was
originally derived by single-cell isolation...
- DNA
fragment has been
cloned into a
cloning vector, it may be
further subcloned into
another vector designed for more
specific use.
There are many types...
-
degree of heterogeneity,
because they
consist of
tumor cells of
multiple subclones,
immune cells, and
other cell types.
Beyond cancer, many gene expression...
