- "selected." In this example, a gene from
mammalian gene
library will be
subcloned into a
bacterial plasmid (destination platform). The
bacterial plasmid...
-
reported in 1973. A neuroblast-like
subclone of SK-N-SH,
named SH-SY, was
subcloned as SH-SY5,
which was
subcloned a
third time to
produce the SH-SY5Y...
- DNA
fragment has been
cloned into a
cloning vector, it may be
further subcloned into
another vector designed for more
specific use.
There are many types...
-
single peptide. As an alternative, scFv can be
created directly from
subcloned heavy and
light chains derived from a hybridoma.
ScFvs have many uses...
- T cloning) is a
subcloning technique that
avoids the use of
restriction enzymes and is
easier and
quicker than
traditional subcloning. The
technique relies...
- genomes.
Cancers usually show
tumour heterogeneity,
containing multiple subclones. They also
frequently have
reduced expression of DNA
repair enzymes due...
-
fragment containing all ****embled
genes to be
excised from the
vector and
subcloned in a next
level of
cloning (Level P).
Level P
vectors are
similar to level...
-
rates of mutation, the high
heterogeneity (variability) of
tumor cell
subclones, and the
absence of
genetic recombination.
Phylogenetics can also aid...
- strand. The
concept is used in
molecular biology, in cloning, or when
subcloning insert DNA into
vector DNA. Such ends may be
generated by restriction...
-
experimental observations. That
particular strand-biased
divergent domain was
subcloned and its
altered helical structure was
studied in
greater detail. A fifth...
