-
molecular biology,
subcloning is a
technique used to move a
particular DNA
sequence from a
parent vector to a
destination vector.
Subcloning is not to be confused...
- were
derived in 1961 by I. A.
Macpherson and M. G. P. Stoker. Nowadays,
subclone 13 is
occasionally used,
which was
originally derived by single-cell isolation...
- 1962 cell line 3T3-J2, a
subclone of 3T3-Swiss albino,
commonly used as
feeders for
keratinocyte cultures 3T3-L1, a
subclone of 3T3-Swiss albino, used...
- genomes.
Cancers usually show
tumour heterogeneity,
containing multiple subclones. They also
frequently have
reduced expression of DNA
repair enzymes due...
- ob/ob mice FBS+ I+ T3 TA1
Subclone of C3H10T1/2 FBS + D + I 30A5
Subclone of C3H10T1/2 FBS + D + M + I 1246
Adipogenic Subclone of CH3
mouse teratocarcinoma...
-
reported in 1973. A neuroblast-like
subclone of SK-N-SH,
named SH-SY, was
subcloned as SH-SY5,
which was
subcloned a
third time to
produce the SH-SY5Y...
- is an
immortalized mouse myoblast cell line. The C2C12 cell line is a
subclone of
myoblasts that were
originally obtained by
Yaffe and
Saxel at the Weizmann...
- may give rise to
subclones that
possess an
evolutionary advantage over the
others within the
tumour environment, and
these subclones may
become dominant...
-
methods common to most
purification tags. The most
basic method is to
subclone the gene of
interest into a
vector containing a
polyhistidine tag sequence...
- T cloning) is a
subcloning technique that
avoids the use of
restriction enzymes and is
easier and
quicker than
traditional subcloning. The
technique relies...
