Definition of SgRNAs. Meaning of SgRNAs. Synonyms of SgRNAs

- CRISPR-Cas9 gene editing system, coupled with libraries of single guide RNAs (sgRNAs), which are designed to target every gene in the genome. Over recent...
- Guide RNA (gRNA) or single guide RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that...
- Depletion or enrichment of sgRNAs is detected and compared to the original sgRNA library, annotated with the target gene that sgRNA corresponds to. Statistical...
- or whole proteins linked to dCas9 or sgRNAs that ****ist in the recruitment of important co-factors as well as RNA Polymerase for transcription of the gene(s)...
- crRNA-tracrRNA complex is replaced by a chimeric single-guide RNA (sgRNA, in red) which has been proved to have the same function as the natural RNA complex...
- Hegde M, Smith I, et al. (December 2014). "Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation". Nature Biotechnology. 32 (12):...
- many sgRNAs simultaneously is to array the sgRNAs in a single construct with multiple promoters or processing elements. For example, Extra-Long sgRNA Arrays...
- the RNA used for targeting Cas9 to a genomic location. Since sgRNAs are usually expressed by RNA polymerase III, which limits the length of the RNA domain...
- its guide RNA are smaller than the protein and RNA components of the Cas9 system; the crRNA of Cas12a is approximately half as long as sgRNAs used with...
- editing and labeling of cells is done by introducing the Cas9 protein and sgRNAs into progenitor cells. The CRISPR-Cas9 complex randomly produces double-stranded...