Definition of SgRNA. Meaning of SgRNA. Synonyms of SgRNA

- Guide RNA (gRNA) or single guide RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that...
- collections of sgRNAs known as sgRNA libraries, or CRISPR knockout libraries, must be generated. The first step in creating a sgRNA library is to identify...
- Guide RNA, CRISPR A small guide RNA (sgRNA), or gRNA is an RNA with around 20 nucleotides used to direct Cas9 or dCas9 to their targets. gRNAs contain...
- crRNA-tracrRNA complex is replaced by a chimeric single-guide RNA (sgRNA, in red) which has been proved to have the same function as the natural RNA complex...
- Depletion or enrichment of sgRNAs is detected and compared to the original sgRNA library, annotated with the target gene that sgRNA corresponds to. Statistical...
- sites into the genomes of progenitor cells. Alongside the barcode, Cas9 and sgRNA are introduced into the cells. Mutations in the barcode ac****ulate during...
- (tracrRNA) is commonly used in experiments and called a single guide RNA (sgRNA). It performs both functions: the first 20 nucleotides of the sgRNA are...
- an RNA-guided manner. Targeting specificity is determined by complementary base-pairing of a single guide RNA (sgRNA) to the genomic locus. sgRNA is a...
- be custom made using tools for sgRNA design, many of which are listed on the CRISPR/cas9 tools Wikipedia page. The sgRNA expression vector design will...
- information that replaces the targeted sequence. The pegRNA consists of an extended single guide RNA (sgRNA) containing a primer binding site (PBS) and a reverse...