-
Sepharose is a
tradename for a crosslinked, beaded-form of agarose, a
polysaccharide polymer material extracted from seaweed. Its
brand name is a portmanteau...
- DEAE-
Sepharose is a
tradename for the anion-exchange
reactive group,
diethylaminoethanol (DEAE)
covalently linked to
Sepharose (a
polysaccharide polymer)...
-
resin or 6
glutamates for
binding to an anion-exchange
resin such as DEAE-
Sepharose).
Elution by
increasing ionic strength of the
mobile phase is more subtle...
- 1-6 10 Bio-gel P-60 3-60 11 Bio-gel P-150 1.5-150 12 Bio-gel P-300 16-400 13
Sepharose 2B 2000-25000 14
Sepharose 4B 300-3000 15
Sepharose 6B 10-20000...
- cross-linked
agarose (
sepharose), sephadex, polyacrylamide, and silica. An
example for
triazine linkage immobilization is Blue
Sepharose,
resulting from Cibacron...
- first-glance
calculation on the cost of
magnetic beads compared to
sepharose beads may make the
sepharose beads appear less expensive. But
magnetic beads may be competitively...
-
cation exchange) SP (sulphopropyl group,
strong cation exchange) DEAE-
Sepharose QFF Ion
exchange resins are the
physical medium that
facilitates ion exchange...
-
carbohydrate molecules that is used in
lectin affinity chromatography are Con A-
Sepharose and WGA-agarose.
Another example of a
lectin is
wheat germ agglutinin...
-
Examples of agarose-based
matrix for gel
filtration chromatography are
Sepharose and
WorkBeads 40 SEC (cross-linked
beaded agarose),
Praesto and Superose...
-
Column chromatography with a
hydrophobic stationary phase such as phenyl-
sepharose will
cause more
hydrophobic proteins to
travel more slowly,
while less...
