-
recognise the double-stranded
sequence RCCGGY and
cleave after the
purine R.
Recognition sequence Cut 5'
RCCGGY 5' ---R CCGGY--- 3' 3'
YGGCCR 3' ---YGGCC...
- Bst31TI, EacI, Ral8I AcoI 11822
Acinetobacter calcoaceticus 5'
YGGCCR 3'
RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' EaeI, Bfi89I, CfrI, EcoHK31I...
- N--- 3' 3' ---CCTAGNNNNN --- 5' EaeI
Enterobacter aerogenes 5'
YGGCCR 3'
RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' Eae46I
Enterobacter aerogenes...
- NNN--- 3' 3' ---GAGAAGNNNN --- 5' Bco118I
Bacillus coagulans 118 5'
RCCGGY 3'
YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'
BcoAI Bacillus coagulans...
- --- 3' 3' ---CGTTAC NN--- 5'
BsrFI Bacillus stearothermophilus CPW16 5'
RCCGGY 3'
YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'
BsrGI Bacillus stearothermophilus...
- TAC--- 3' 3' ---CAT ATG--- 5' BstB7SI
Bacillus stearothermophilus B7S 5'
RCCGGY 3'
YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' BstBS32I Bacillus...
- Kzo9I, NdeII,
SsiBI Bfi89I
Butyrivibrio fibrisolvens OB189 5'
YGGCCR 3'
RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' BflI
Bacillus flavothermus 5'...
- HinP1I, HsoI, HspAI,
SciNI CfrI
Citrobacter freundii RFL2 5'
YGGCCR 3'
RCCGGY 5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5' Cfr6I
Citrobacter freundii RFL6...
- Bse118I
Bacillus sp. 118 5'
RCCGGY 3'
YGGCCR 5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' Bse634I 1KNV
Bacillus sp. 634 5'
RCCGGY 3'
YGGCCR 5' ---R CCGGY---...
