-
aspects of DNA repair.
Defects in
certain nucleases can
cause genetic instability or immunodeficiency.
Nucleases are also
extensively used in
molecular cloning...
-
major classes of
these enzymes—zinc
finger nucleases (ZFNs),
transcription activator-like
effector nucleases (TALENs) and
engineered meganucleases—were...
- a
technique known as
genome editing with
engineered nucleases.
Alongside zinc
finger nucleases and CRISPR/Cas9,
TALEN is a
prominent tool in the field...
- of
engineered nucleases including engineered homing endonucleases and
nucleases based on
engineered TAL effectors. TAL
effector nucleases (TALENs) are...
- for a
specific sequence is an area of
active research, and zinc
finger nucleases and zinc
finger transcription factors are two of the most
important applications...
- (indels).
Surveyor nuclease is part of a
family of mismatch-specific
endonucleases that were
discovered in
celery (CEL
nucleases). The
enzyme recognizes...
-
Kandavelou K, Wu J,
Porteus M,
Chandrasegaran S (2005). "Zinc
finger nucleases: custom-designed
molecular scissors for
genome engineering of
plant and...
- CRISPR-Cas9 and CRISPR-Cpf1,
there are
doubtless many yet
undiscovered nucleases and PAMs. CRISPR/Cas13a (formerly C2c2) from the
bacterium Leptotrichia...
- in lysosomes,
which are
acidic on the inside. Acid
hydrolases may be
nucleases, proteases, glycosidases, lipases, phosphatases,
sulfatases and phospholipases...
- bp) and can be
engineered to bind to
desired DNA sequences. Zinc
finger nucleases are the most
commonly used
artificial restriction enzymes and are generally...
