- In biochemistry, a
nuclease (also
archaically known as
nucleodepolymerase or polynucleotidase) is an
enzyme capable of
cleaving the
phosphodiester bonds...
-
Nuclease S1 (EC 3.1.30.1) is an
endonuclease enzyme that
splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This
enzyme catalyses...
-
current nuclease-based gene
editing platforms but its use was
limited by low
efficiencies of editing.
Genome editing with
engineered nucleases, i.e. all...
-
Micrococcal nuclease (EC 3.1.31.1, S7
Nuclease, MNase,
spleen endonuclease, thermonuclease,
nuclease T,
micrococcal endonuclease,
nuclease T', staphylococcal...
-
Transcription activator-like
effector nucleases (TALEN) are
restriction enzymes that can be
engineered to cut
specific sequences of DNA. They are made...
- Zinc-finger
nucleases (ZFNs) are
artificial restriction enzymes generated by
fusing a zinc
finger DNA-binding
domain to a DNA-cleavage domain. Zinc finger...
-
Surveyor nuclease ****ay is an
enzyme mismatch cleavage ****ay used to
detect single base
mismatches or
small insertions or
deletions (indels). Surveyor...
- MNase-seq,
short for
micrococcal nuclease digestion with deep sequencing, is a
molecular biological technique that was
first pioneered in 2006 to measure...
- Crab duplex-specific
nuclease is a
nuclease derived from the red king crab (Paralithodes camtschaticus,
Kamchatka crab)
hepatopancreas that
displays a...
-
helices α4 and α5 and two
loops P1 and P2 of the
cleavage domain. When the
nuclease is
unbound to DNA, the
endonuclease domain is
sequestered by the DNA-binding...
