- In biochemistry, a
nuclease (also
archaically known as
nucleodepolymerase or polynucleotidase) is an
enzyme capable of
cleaving the
phosphodiester bonds...
-
current nuclease-based gene-editing platforms, but its use was
limited by low
efficiencies of editing.
Genome editing with
engineered nucleases, i.e. all...
- Zinc-finger
nucleases (ZFNs) are
artificial restriction enzymes generated by
fusing a zinc
finger DNA-binding
domain to a DNA-cleavage domain. Zinc finger...
-
Transcription activator-like
effector nucleases (TALEN) are
restriction enzymes that can be
engineered to cut
specific sequences of DNA. They are made...
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Micrococcal nuclease (EC 3.1.31.1, S7
Nuclease, MNase,
spleen endonuclease, thermonuclease,
nuclease T,
micrococcal endonuclease,
nuclease T', staphylococcal...
-
unintended genetic modifications that can
arise through the use of
engineered nuclease technologies such as: clustered,
regularly interspaced,
short palindromic...
-
Serratia marcescens nuclease (EC 3.1.30.2,
endonuclease (Serratia marcescens),
barley nuclease,
plant nuclease I,
nucleate endonuclease) is an enzyme....
- Mung bean
nuclease (
Nuclease MB) is a
nuclease derived from
sprouts of the mung bean (Vigna radiata) that
removes nucleotides in a step-wise
manner from...
-
Surveyor nuclease ****ay is an
enzyme mismatch cleavage ****ay used to
detect single base
mismatches or
small insertions or
deletions (indels). Surveyor...
-
helices α4 and α5 and two
loops P1 and P2 of the
cleavage domain. When the
nuclease is
unbound to DNA, the
endonuclease domain is
sequestered by the DNA-binding...
