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Limulus amebocyte lysate (LAL) is an
aqueous extract of
motile blood cells (amebocytes) from the
Atlantic horseshoe crab
Limulus polyphemus. LAL reacts...
- its integrity. A
fluid containing the
contents of
lysed cells is
called a
lysate. In
molecular biology, biochemistry, and cell
biology laboratories, cell...
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Human platelet lysate (or hPL) is a
substitute supplement for
fetal bovine serum (FBS) in
experimental and
clinical cell culture. It is a turbid, light-yellow...
- The
blood of
horseshoe crabs contains a
clotting agent,
Limulus Amebocyte Lysate,
which is now used to test that
antibiotics and
kidney machines are free...
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global bacterial diseases.
Vaccines made from
attenuated whole cells or
lysates have been
replaced largely by less reactogenic, cell-free
vaccines consisting...
-
anything bound to them (co-immunoprecipitation) from
other molecules in a cell
lysate, in
Western blot
analyses to
identify proteins separated by electrop****sis...
- Tris-HCl) and
ionic salts (e.g. NaCl) to
regulate the pH and
osmolarity of the
lysate.
Sometimes detergents (such as
Triton X-100 or SDS) are
added to
break up...
-
crabs are
often caught for
their blood,
which contains Limulus amebocyte lysate, a
chemical used to
detect bacterial endotoxins. Additionally, the animals...
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different proteins.
These solutions will
often be in the form of a
crude lysate of a
plant or
animal tissue.
Other sample types could be body
fluids or...
-
complex samples, such as
tissue lysates.
Cells are
isolated from
various tissues of
interest and are lysed. The
lysate is arra**** onto the
microarray and...