- its integrity. A
fluid containing the
contents of
lysed cells is
called a
lysate. In
molecular biology, biochemistry, and cell
biology laboratories, cell...
-
Limulus amebocyte lysate (LAL) is an
aqueous extract of
motile blood cells (amebocytes) from the
Atlantic horseshoe crab
Limulus polyphemus. LAL reacts...
-
Human platelet lysate (or hPL) is a
substitute supplement for
fetal bovine serum (FBS) in
experimental and
clinical cell culture. It is a turbid, light-yellow...
-
different experiments. The
procedure begins by
making a cell
lysate of the
cells of interest. This
lysate contains polysomes,
monosomes (composed of one ribosome...
-
crabs are
often caught for
their blood,
which contains Limulus amebocyte lysate, a
chemical used to
detect bacterial endotoxins. Additionally, the animals...
-
disrupted and its
internal contents released into a
solution known as a
crude lysate. The
resulting mixture can be
purified using ultracentrifugation, which...
-
different proteins.
These solutions will
often be in the form of a
crude lysate of a
plant or
animal tissue.
Other sample types could be body
fluids or...
- A
reverse phase protein lysate microarray (RPMA) is a
protein microarray designed as a dot-blot
platform that
allows measurement of
protein expression...
- The
blood of
horseshoe crabs contains a
clotting agent,
Limulus Amebocyte Lysate,
which is now used to test that
antibiotics and
kidney machines are free...
- Tris-HCl) and
ionic salts (e.g. NaCl) to
regulate the pH and
osmolarity of the
lysate.
Sometimes detergents (such as
Triton X-100 or SDS) are
added to
break up...
