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Recognition Sequence Cut Site 5'CTGCAG 3' 3'
GACGTC 5' 5'--CTGCA G--3' 3'--G ACGTC—5'...
- 5'---GGTAC C---3' 3'---C CATGG---5' PstI
Providencia stuartii 5'CTGCAG 3'
GACGTC 5'---CTGCA G---3' 3'---G ACGTC---5' SacI
Streptomyces achromogenes 5'GAGCTC...
- Eco1524I, PceI, Pme55I, SarI, Sru30DI, SteI
AatII 7
Acetobacter aceti 5'
GACGTC 3'
CTGCAG 5' ---GACGT C--- 3' 3' ---C TGCAG--- 5' Ssp5230I, ZraI AauI...
- high
binding affinity for the ACGT motifs,
which include CACGTG (G box),
GACGTC (C box),
TACGTA (A box),
AACGTT (T box), and a GCN4 motif,
namely TGA(G/C)TCA...
- 3' 3' ---C YCGRG--- 5'
PaeHII Pseudomonas aeruginosa 4148 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AliAJI, BspBI, BspMAI, CfuII...
- TCGAC--- 3' 3' ---CAGCT G--- 5'
SalPI Streptomyces albus 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' ApiI, BspMAI, CstI, Ecl37kI...
- CCNGG--- 3' 3' ---GGNCC --- 5' Ecl37kI
Enterobacter cloacae 37k 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, Ecl2zI, PaePI...
- Bsu1532I, FalII, FnuDII, MvnI, ThaI Bsp63I
Bacillus sphaericus 63 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, Asp713I, BloHII, MhaAI...
- 3' ---YCTAG R--- 5'
BloHII Bifidobacterium longum E194b 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, Asp713I, BsuBI, CflI,...
- AATTC--- 3' 3' ---CTTAA G--- 5'
HalII Hafnia alvei B6 5'
CTGCAG 3'
GACGTC 5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5' AjoI, AliAJI, Bsp63I, CfrA4I...
