-
formazans are
compounds of the
general formula [R-N=N-C(R')=N-NH-R"],
formally derivatives of
formazan [H2NN=CHN=NH],
unknown in free form.
Formazan dyes...
- 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, to its
insoluble formazan,
which has a
purple color.
Other closely related tetrazolium dyes including...
-
colorimetric reaction.
Viable cells will
reduce the MTS
reagent to a
colored formazan product. A
similar redox-based ****ay has also been
developed using the...
-
depends upon the
direct reduction of NBT to the
insoluble blue
compound formazan by
superoxide which is
produced by
normal peripheral blood neutrophils...
-
Dehydrogenase enzymes present in
living tissue reduce the
tetrazolium chloride to
formazan, a reddish, water-insoluble compound. This
reaction occurs in or near living...
- speeds.
There are many
hybrid variations of the dye formulations, such as
Formazan by
Kodak (a
hybrid of
cyanine and phthalocyanine). Many
manufacturers have...
- ****ays show
excellent correlation to
reference viability ****ays such as
formazan-based ****ays (MTT/XTT) and
tritiated thymidine based techniques. The low...
- 7-Aminoactinomycin D (7-AAD).
Mitochondrial activity or caspase:
Resazurin and
Formazan (MTT/XTT) can ****ay for
various stages in the
apoptosis process that foreshadows...
- ions),
similar to
tetrazolium chloride that on
reduction produces a red
formazan dye that can be used for
quantitative redox ****ays. It is also
toxic to...
-
reduction of
yellow tetrazolium salt (MTT) by
active cells to
produce purple formazan crystals which ac****ulate in
living cells.
Cells are lysed, the crystals...
