-
typically called restriction endonucleases or
restriction enzymes,
cleave only at very
specific nucleotide sequences.
Endonucleases differ from exonucleases...
- Type IIM
restriction endonucleases, such as DpnI, are able to
recognize and cut
methylated DNA. Type IIS
restriction endonucleases (e.g. FokI)
cleave DNA...
- four
types of AP
endonucleases that have been
classified according to
their mechanism and site of incision.
class I AP
endonucleases (EC 4.2.99.18) cleave...
- The
homing endonucleases are a
collection of
endonucleases encoded either as
freestanding genes within introns, as
fusions with host proteins, or as self-splicing...
- structure-catalysis
relationship of type II
endonucleases, site-directed
mutagenesis of the
restriction endonuclease HindIII has
provided much
insight into...
- fragments. Not all
restriction endonucleases cut
symmetrically and
leave blunt ends like
HindII described above. Many
endonucleases cleave the DNA backbones...
- Flap
endonucleases (FENs, also
known as 5'
durgs in
older references) are a
class of
nucleolytic enzymes that act as both 5'-3'
exonucleases and structure-specific...
-
Nuclease S1 (EC 3.1.30.1) is an
endonuclease enzyme that
splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This
enzyme catalyses...
- (EC 3.1.31.1, S7 Nuclease, MNase,
spleen endonuclease, thermonuclease,
nuclease T,
micrococcal endonuclease,
nuclease T',
staphylococcal nuclease, spleen...
-
contains the PD..D/EXK
motif within its
active site like many
restriction endonucleases. The
enzyme is a
homodimer of a 31
kilodalton subunit consisting of...
