- end.
These new constructs,
called ditags, are then PCR
amplified using anchor A and B
specific primers. The
ditags are then
cleaved using the original...
-
ENCODE subgroups including RNASeq data,
histone modification and CAGE and
Ditag data.
RNAseq data is an
important new
source of evidence, but generating...
- SAGE,
concatenation takes place after ditags are
formed and
amplified by PCR. The
linkers on the
outside of the
ditags are
cleaved with the
enzyme that provided...
- namely: SAGE:
serial analysis of gene
expression (ChIP-SAGE), PET:
paired end
ditag sequencing (ChIP-PET) and more recently, next-generation
sequencing (ChIP-Seq)...
-
obtained via
restriction enzyme digests.
These tags are then
linked to into
ditags, concatenated, cloned,
sequenced and
mapped back to the
reference genome...
- Paired-end tags (PET) (sometimes "Paired-End diTags", or
simply "
ditags") are the
short sequences at the 5’ and 3' ends of a DNA
fragment which are unique...
