- on the
ribose lack
hydroxyl groups, and are
abbreviated as
ddNTPs (
ddGTP,
ddATP,
ddTTP and
ddCTP). The
Sanger method is used to
amplify a
target segment...
- DNA
polymerase to
cease extension of DNA when a
modified ddNTP is incorporated. The
ddNTPs may be
radioactively or
fluorescently labelled for detection...
-
overabundance of
ddNTPs will
create multiple fragments of the same size and
position of the DNA template. When the DNA
polymerase adds a
ddNTP the fragment...
-
causing DNA
polymerase to
cease extension of DNA when a
ddNTP is incorporated. The
ddNTPs may be
radioactively or
fluorescently labelled for detection...
-
fluorescently labeled dideoxynucleotides (
ddNTP) or
fluorescently labeled deoxynucleotides (d
NTP). With
ddNTPs,
probes hybridize to the
target DNA immediately...
-
common form of
nucleic acid
detection that may use
fluorescently labeled ddNTPs to
image fluorescence peaks Fluorescence image guided surgery: is a medical...
-
staining and
scanning of chip
After incorporation of
these hapten-labeled
ddNTPs, multi-layered
immunohistochemical ****ays are
performed by
repeated rounds...
-
determine this C:T ratio. At the beginning, MS-SnuPE
relied on
radioactive ddNTPs as the
reporter of the
primer extension. Fluorescence-based
methods or Pyrosequencing...
- example, E. coli DnaG will use 2',3'-dideoxynucleoside 5'-triphosphates (
ddNTPs) as substrates,
which act as
chain terminators due to the lack of a 3' hydroxyl...
- the
concept of leap seconds:
NTP specifies a flag to
inform the
receiver that a leap
second is imminent. However, some
NTP server implementations have...
