- on the
ribose lack
hydroxyl groups, and are
abbreviated as
ddNTPs (
ddGTP,
ddATP,
ddTTP and
ddCTP). The
Sanger method is used to
amplify a
target segment...
- (dATP, d
GTP, dCTP and dTTP) and the DNA polymerase. To each
reaction is
added only one of the four
dideoxynucleotides (
ddATP,
ddGTP,
ddCTP, or
ddTTP), while...
-
extension with hapten-labeled dideoxynucleotides. The
ddCTP and
ddGTP are
labeled with
biotin while ddATP and
ddUTP are
labeled with 2,4-dinitrophenol (DNP). Fluorescence...
- bind to the
nucleotide guanosine triphosphate (
GTP) and
hydrolyze it to
guanosine diphosphate (GDP). The
GTP binding and
hydrolysis takes place in the highly...
- ‘tailing’ activity' to add a chain-terminating
dideoxynucleotide base,
ddRTP (
ddATP or
ddGTP), to the 3’ end of the IT, in the
presence of
manganese (Mn2+)....
-
guanosine diphosphate (GDP) to
allow binding of
guanosine triphosphate (
GTP). A
variety of
unrelated structural domains have been
shown to
exhibit guanine...
- The Gs
alpha subunit of the
stimulated G
protein complex exchanges GDP for
GTP and is
released from the complex. In a cAMP-dependent pathway, the activated...
-
Toyota G
Sports Martin, J.A.; Fuller, M.J. (2008).
Inside IMSA's
Legendary GTP Race Cars: The
Prototype Experience. US: Motorbooks. "Toyota
Motorsport GmbH"...
- G-protein
transducin (Gt),
resulting in the
liberation of its α subunit. This
GTP-bound
subunit in turn
activates a cGMP phosphodiesterase. The cGMP phosphodiesterase...
-
downstream region. The P-loop is best
known for its
presence in ATP- and
GTP-binding proteins, and is also
found in a
variety of
proteins with phosphorylated...