-
bottom strand reads 3'-
CTTAAG-5'. If the DNA
strand is
flipped over, the
sequences are
exactly the same (5'GAATTC-3' and 3'-
CTTAAG-5'). Here are more restriction...
-
Enzyme Source Recognition Sequence Cut
EcoRI Escherichia coli 5'GAATTC 3'
CTTAAG 5'---G AATTC---3' 3'---CTTAA G---5'
EcoRII Escherichia coli 5'CCWGG 3'GGWCC...
- 3'–TTCGAA–5' 5'–A AGCTT–3' 3'–TTCGA A–5'
EcoRI Escherichia coli 5'–GAATTC-3' 3'–
CTTAAG–5' 5'–G AATTC–3' 3'–CTTAA G–5'
BamHI Bacillus amyloliquefaciens 5'–GGATCC–3'...
- 5' ---C TRYAG--- 3' 3' ---****RT C--- 5' BfrI
Bacteroides fragilis 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
BfrBI Bacteroides...
- 32 5'
GAATTC 3'
CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' Srl55DI
Selenomonas ruminantium lactilytica 55 5'
GAATTC 3'
CTTAAG 5' ---G AATTC---...
- BshNI, BspT107I, Eco64I, HgiCI, HgiHI,
PfaAI MspCI Micrococcus sp. 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' MspR9I Micrococcus...
- Mph1103I, NsiI, SepI, SspD5II Ppu111I
Pseudomonas putida P111 5'
GAATTC 3'
CTTAAG 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
PpuAI Pseudomonas putida A1...
- BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI
AflII 39
Anabaena flos-aquae 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' BfrI, BspTI, Bst98I...
- NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5'
BspTI Bacillus sp. RFL1265 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
AflII BspT104I Bacillus...
- AcpII, Asp10HII, BasI, Esp1396I, PflBI,
PflMI Vha464I
Vibrio harveyi 464 5'
CTTAAG 3'
GAATTC 5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' VneI
Vibrio nereis...
