- II
restriction enzyme EC that
recognise the double-stranded DNA
sequence CTCGAG and
cleaves after C-1. Type II
restriction endonucleases (EC) are components...
- 5'---CTGCA G---3' 3'---G ACGTC---5' SacI
Streptomyces achromogenes 5'GAGCTC 3'
CTCGAG 5'---GAGCT C---3' 3'---C TCGAG---5' SalI
Streptomyces albus 5'GTCGAC 3'CAGCTG...
-
sequence Cut
Isoschizomers SacI
Streptomyces achromogenes 5'
GAGCTC 3'
CTCGAG 5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5'
SacII Streptomyces achromogenes...
- TCAGC--- 3' 3' ---GGAGT CG--- 5'
BbvCI AbrI 8
Azospirillum brasilense 5'
CTCGAG 3'
GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BluI, BspAAI, BssHI...
- ---GATNN NNATC--- 3' 3' ---CTANN NNTAG--- 5' MavI
Mycobacterium avium 5'
CTCGAG 3'
GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI...
- and
BglII sites (GAATTCaaaAGATCT) and 3′
BamHI and XhoI
sites (GGATCCaaa
CTCGAG), and
lacking in
these same
restriction sites internally. The
upstream part...
- 3' ---GTC GAC--- 5' Ecl136II
Enterobacter cloacae RFL136 5'
GAGCTC 3'
CTCGAG 5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'
EclHKI Enterobacter cloacae...
- GG--- 3' 3' ---GG CGCC--- 5' PaeR7I
Pseudomonas aeruginosa PA0303 pMG7 5'
CTCGAG 3'
GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI...
-
GTCGAC 5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
BspAAI Bacillus sp. AA 5'
CTCGAG 3'
GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BstVI...
- BstFNI, BstUI, Csp68KVI, FalII,
FnuDII TliI
Thermococcus litoralis 5'
CTCGAG 3'
GAGCTC 5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' AbrI, BluI, BssHI...